A comparative evaluation of the metabolic profiles of normal and inflammatory knee-joint synovial fluids by high resolution proton NMR spectroscopy

Declan P. Naughton; Rachel Haywood; David R. Blake; Sally Edwards; Geoffrey Hawkes; Martin Grootveld

doi:10.1016/0014-5793(93)80636-9

A comparative evaluation of the metabolic profiles of normal and inflammatory knee-joint synovial fluids by high resolution proton NMR spectroscopy

Declan P. Naughton
, Rachel Haywood
, David R. Blake
, Sally Edwards
, Geoffrey Hawkes
, Martin Grootveld

Research output: Contribution to journal › Article › peer-review

Abstract

High resolution 1H NMR spectroscopy has been employed to investigate the metabolic profile of healthy human knee-joint synovial fluid (SF) and the biochemical data acquired have been compared with those of matched serum, and inflammatory knee-joint SF samples. Results obtained indicate that the healthy human knee-joint has a hypoxic status (high lactate level when expressed relative to that of paired serum) that is milder than that of the inflamed human knee-joint. Moreover, normal SF differs from that of inflammatory SF in that it contains little or no NMR-detectable lipoprotein-associated fatty acids and 'acute-phase' glycoproteins, an observation reflecting the limited passage of these macromolecules from plasma into the synovial space in healthy subjects.

Original language	English
Pages (from-to)	221-225
Journal	FEBS Letters
Volume	332
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)80636-9
Publication status	Published - 18 Oct 1993

Bibliographical note

Note: This work was supported by the Arthritis and Rheumatism Council (UK) [grant number B0141] and the Ministry of Agriculture, Fisheries and Food (UK).

Keywords

Allied health professions and studies

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10.1016/0014-5793(93)80636-9

http://dx.doi.org/10.1016/0014-5793(93)80636-9

Cite this

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title = "A comparative evaluation of the metabolic profiles of normal and inflammatory knee-joint synovial fluids by high resolution proton NMR spectroscopy",

abstract = "High resolution 1H NMR spectroscopy has been employed to investigate the metabolic profile of healthy human knee-joint synovial fluid (SF) and the biochemical data acquired have been compared with those of matched serum, and inflammatory knee-joint SF samples. Results obtained indicate that the healthy human knee-joint has a hypoxic status (high lactate level when expressed relative to that of paired serum) that is milder than that of the inflamed human knee-joint. Moreover, normal SF differs from that of inflammatory SF in that it contains little or no NMR-detectable lipoprotein-associated fatty acids and 'acute-phase' glycoproteins, an observation reflecting the limited passage of these macromolecules from plasma into the synovial space in healthy subjects.",

keywords = "Allied health professions and studies",

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note = "Note: This work was supported by the Arthritis and Rheumatism Council (UK) [grant number B0141] and the Ministry of Agriculture, Fisheries and Food (UK).",

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doi = "10.1016/0014-5793(93)80636-9",

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T1 - A comparative evaluation of the metabolic profiles of normal and inflammatory knee-joint synovial fluids by high resolution proton NMR spectroscopy

AU - Naughton, Declan P.

AU - Haywood, Rachel

AU - Blake, David R.

AU - Edwards, Sally

AU - Hawkes, Geoffrey

AU - Grootveld, Martin

N1 - Note: This work was supported by the Arthritis and Rheumatism Council (UK) [grant number B0141] and the Ministry of Agriculture, Fisheries and Food (UK).

PY - 1993/10/18

Y1 - 1993/10/18

N2 - High resolution 1H NMR spectroscopy has been employed to investigate the metabolic profile of healthy human knee-joint synovial fluid (SF) and the biochemical data acquired have been compared with those of matched serum, and inflammatory knee-joint SF samples. Results obtained indicate that the healthy human knee-joint has a hypoxic status (high lactate level when expressed relative to that of paired serum) that is milder than that of the inflamed human knee-joint. Moreover, normal SF differs from that of inflammatory SF in that it contains little or no NMR-detectable lipoprotein-associated fatty acids and 'acute-phase' glycoproteins, an observation reflecting the limited passage of these macromolecules from plasma into the synovial space in healthy subjects.

AB - High resolution 1H NMR spectroscopy has been employed to investigate the metabolic profile of healthy human knee-joint synovial fluid (SF) and the biochemical data acquired have been compared with those of matched serum, and inflammatory knee-joint SF samples. Results obtained indicate that the healthy human knee-joint has a hypoxic status (high lactate level when expressed relative to that of paired serum) that is milder than that of the inflamed human knee-joint. Moreover, normal SF differs from that of inflammatory SF in that it contains little or no NMR-detectable lipoprotein-associated fatty acids and 'acute-phase' glycoproteins, an observation reflecting the limited passage of these macromolecules from plasma into the synovial space in healthy subjects.

KW - Allied health professions and studies

UR - http://www.ncbi.nlm.nih.gov/pubmed/7691662

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DO - 10.1016/0014-5793(93)80636-9

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SP - 221

EP - 225

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

A comparative evaluation of the metabolic profiles of normal and inflammatory knee-joint synovial fluids by high resolution proton NMR spectroscopy

Abstract

Bibliographical note

Keywords

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