A rapid LC-MS method for determination of plasma anion profiles of acidotic patients

William McKinnon; Gwyn A. Lord; Lui G. Forni; Jean Marie R. Peron; Philip J. Hilton

doi:10.1016/j.jchromb.2006.01.025

A rapid LC-MS method for determination of plasma anion profiles of acidotic patients

William McKinnon
, Gwyn A. Lord
, Lui G. Forni
, Jean Marie R. Peron
, Philip J. Hilton

Research output: Contribution to journal › Article › peer-review

Abstract

In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis.

Original language	English
Pages (from-to)	179-185
Journal	Journal of Chromatography B
Volume	833
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2006.01.025
Publication status	Published - 3 Apr 2006

Keywords

Other laboratory based clinical subjects

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10.1016/j.jchromb.2006.01.025

http://dx.doi.org/10.1016/j.jchromb.2006.01.025

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title = "A rapid LC-MS method for determination of plasma anion profiles of acidotic patients",

abstract = "In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis.",

keywords = "Other laboratory based clinical subjects",

author = "William McKinnon and Lord, \{Gwyn A.\} and Forni, \{Lui G.\} and Peron, \{Jean Marie R.\} and Hilton, \{Philip J.\}",

year = "2006",

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doi = "10.1016/j.jchromb.2006.01.025",

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volume = "833",

pages = "179--185",

journal = "Journal of Chromatography B",

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TY - JOUR

T1 - A rapid LC-MS method for determination of plasma anion profiles of acidotic patients

AU - McKinnon, William

AU - Lord, Gwyn A.

AU - Forni, Lui G.

AU - Peron, Jean Marie R.

AU - Hilton, Philip J.

PY - 2006/4/3

Y1 - 2006/4/3

N2 - In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis.

AB - In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis.

KW - Other laboratory based clinical subjects

UR - http://www.ncbi.nlm.nih.gov/pubmed/16524788

U2 - 10.1016/j.jchromb.2006.01.025

DO - 10.1016/j.jchromb.2006.01.025

M3 - Article

C2 - 16524788

SN - 1570-0232

VL - 833

SP - 179

EP - 185

JO - Journal of Chromatography B

JF - Journal of Chromatography B

IS - 2

ER -

A rapid LC-MS method for determination of plasma anion profiles of acidotic patients

Abstract

Keywords

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