TY - JOUR
T1 - A rapid LC-MS method for determination of plasma anion profiles of acidotic patients
AU - McKinnon, William
AU - Lord, Gwyn A.
AU - Forni, Lui G.
AU - Peron, Jean Marie R.
AU - Hilton, Philip J.
PY - 2006/4/3
Y1 - 2006/4/3
N2 - In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis.
AB - In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis.
KW - Other laboratory based clinical subjects
UR - http://www.ncbi.nlm.nih.gov/pubmed/16524788
U2 - 10.1016/j.jchromb.2006.01.025
DO - 10.1016/j.jchromb.2006.01.025
M3 - Article
C2 - 16524788
SN - 1570-0232
VL - 833
SP - 179
EP - 185
JO - Journal of Chromatography B
JF - Journal of Chromatography B
IS - 2
ER -