'Campylobacter jejuni' gene cj0511 encodes a serine peptidase essential for colonisation

A.V. Karlyshev; G. Thacker; M.A. Jones; M.O. Clements; B.W. Wren

doi:10.1016/j.fob.2014.04.012

'Campylobacter jejuni' gene cj0511 encodes a serine peptidase essential for colonisation

A.V. Karlyshev, G. Thacker, M.A. Jones, M.O. Clements, B.W. Wren

Research output: Contribution to journal › Article › peer-review

Abstract

According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.

Original language	English
Pages (from-to)	468-472
Journal	FEBS Open Bio
Volume	4
DOIs	https://doi.org/10.1016/j.fob.2014.04.012
Publication status	Published - May 2014

Keywords

Allied health professions and studies

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10.1016/j.fob.2014.04.012

Karlyshev-A-28472Final published version, 698 KBLicence: CC BY-NC-ND

http://dx.doi.org/10.1016/j.fob.2014.04.012

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title = "'Campylobacter jejuni' gene cj0511 encodes a serine peptidase essential for colonisation",

abstract = "According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.",

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author = "A.V. Karlyshev and G. Thacker and M.A. Jones and M.O. Clements and B.W. Wren",

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T1 - 'Campylobacter jejuni' gene cj0511 encodes a serine peptidase essential for colonisation

AU - Karlyshev, A.V.

AU - Thacker, G.

AU - Jones, M.A.

AU - Clements, M.O.

AU - Wren, B.W.

PY - 2014/5

Y1 - 2014/5

N2 - According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.

AB - According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.

KW - Allied health professions and studies

UR - http://www.ncbi.nlm.nih.gov/pubmed/24918062

U2 - 10.1016/j.fob.2014.04.012

DO - 10.1016/j.fob.2014.04.012

M3 - Article

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SN - 2211-5463

VL - 4

SP - 468

EP - 472

JO - FEBS Open Bio

JF - FEBS Open Bio

ER -

'Campylobacter jejuni' gene cj0511 encodes a serine peptidase essential for colonisation

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