Development of molecularly imprinted polymers (MIPs) - assisted sensitive analytical methodologies for detection and monitoring of protein biomarkers

Molecularly imprinted polymers (MIPs) are synthetic polymers with "structural memory", designed to bind target molecules with exceptional selectivity. Their unique properties make them valuable in medicine, chemistry, analysis, and sensing, particularly for recognising biomarkers which are measurable indicators of biological and pathological processes. This thesis explores MIPs for biomarker analysis, focusing on pepsin, a protein biomarker linked to gastroesophageal reflux disease (GERD). This thesis includes a comprehensive investigation into various MIP types, synthesis methods, and applications. Practical work involved developing different MIP systems for pepsin enzyme analysis.