Differential expression of CD180 and IgM by B-cell chronic lymphocytic leukaemia cells using mutated and unmutated immunoglobulin VH genes

N. Kulikova; A.P. Jewell; N. Porakishvili; P.Y. Youinou; K. Yong; A. Nathwani; B. Heelan; V. Duke; T.J. Hamblin; P. Wallace; P. Ely; E.A. Clark; P.M. Lydyard

doi:10.1111/j.1365-2141.2005.05775.x

Differential expression of CD180 and IgM by B-cell chronic lymphocytic leukaemia cells using mutated and unmutated immunoglobulin VH genes

N. Kulikova, A.P. Jewell, N. Porakishvili, P.Y. Youinou, K. Yong, A. Nathwani, B. Heelan, V. Duke, T.J. Hamblin, P. Wallace, P. Ely, E.A. Clark, P.M. Lydyard

Research output: Contribution to journal › Article › peer-review

Abstract

We have studied the surface expression of the Toll-like receptor family member CD 180 on cells from 78 patients with B-chronic lymphocytic leukaemia (B-CLL). B-CLL cells had variable levels of CD 180 expression, but this was always less than that expressed by normal blood B cells and was stable for 24 months. Significantly higher levels of CD 180 were expressed by B-CLL cells with mutated IGVH genes compared with those using unmutated IGVH genes. This was in contrast to the higher levels of expression of surface immunoglobulin M by B-CLL cells using unmutated, rather than mutated IGVH genes. CD 180 was functional on B-CLL cells from some of the patients, as shown by the increased expression of CD 86 following incubation in vitro with anti-CD 180. The differential expression of CD 180 amongst B-CLL patients is one more marker that may define more precisely the different biological properties of this heterogeneous disease.

Original language	English
Pages (from-to)	313-319
Journal	British Journal of Haematology
Volume	131
Issue number	3
DOIs	https://doi.org/10.1111/j.1365-2141.2005.05775.x
Publication status	Published - Nov 2005
Externally published	Yes

Bibliographical note

Note: This work was supported by the Leukaemia Research Fund and INTAS EU [grant number 01-2239].

Keywords

Cancer studies

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10.1111/j.1365-2141.2005.05775.x

http://dx.doi.org/10.1111/j.1365-2141.2005.05775.x

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Kulikova, N., Jewell, A. P., Porakishvili, N., Youinou, P. Y., Yong, K., Nathwani, A., Heelan, B., Duke, V., Hamblin, T. J., Wallace, P., Ely, P., Clark, E. A., & Lydyard, P. M. (2005). Differential expression of CD180 and IgM by B-cell chronic lymphocytic leukaemia cells using mutated and unmutated immunoglobulin VH genes. British Journal of Haematology, 131(3), 313-319. https://doi.org/10.1111/j.1365-2141.2005.05775.x

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title = "Differential expression of CD180 and IgM by B-cell chronic lymphocytic leukaemia cells using mutated and unmutated immunoglobulin VH genes",

abstract = "We have studied the surface expression of the Toll-like receptor family member CD 180 on cells from 78 patients with B-chronic lymphocytic leukaemia (B-CLL). B-CLL cells had variable levels of CD 180 expression, but this was always less than that expressed by normal blood B cells and was stable for 24 months. Significantly higher levels of CD 180 were expressed by B-CLL cells with mutated IGVH genes compared with those using unmutated IGVH genes. This was in contrast to the higher levels of expression of surface immunoglobulin M by B-CLL cells using unmutated, rather than mutated IGVH genes. CD 180 was functional on B-CLL cells from some of the patients, as shown by the increased expression of CD 86 following incubation in vitro with anti-CD 180. The differential expression of CD 180 amongst B-CLL patients is one more marker that may define more precisely the different biological properties of this heterogeneous disease.",

keywords = "Cancer studies",

author = "N. Kulikova and A.P. Jewell and N. Porakishvili and P.Y. Youinou and K. Yong and A. Nathwani and B. Heelan and V. Duke and T.J. Hamblin and P. Wallace and P. Ely and E.A. Clark and P.M. Lydyard",

note = "Note: This work was supported by the Leukaemia Research Fund and INTAS EU [grant number 01-2239].",

year = "2005",

month = nov,

doi = "10.1111/j.1365-2141.2005.05775.x",

language = "English",

volume = "131",

pages = "313--319",

journal = "British Journal of Haematology",

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Kulikova, N, Jewell, AP, Porakishvili, N, Youinou, PY, Yong, K, Nathwani, A, Heelan, B, Duke, V, Hamblin, TJ, Wallace, P, Ely, P, Clark, EA & Lydyard, PM 2005, 'Differential expression of CD180 and IgM by B-cell chronic lymphocytic leukaemia cells using mutated and unmutated immunoglobulin VH genes', British Journal of Haematology, vol. 131, no. 3, pp. 313-319. https://doi.org/10.1111/j.1365-2141.2005.05775.x

TY - JOUR

T1 - Differential expression of CD180 and IgM by B-cell chronic lymphocytic leukaemia cells using mutated and unmutated immunoglobulin VH genes

AU - Kulikova, N.

AU - Jewell, A.P.

AU - Porakishvili, N.

AU - Youinou, P.Y.

AU - Yong, K.

AU - Nathwani, A.

AU - Heelan, B.

AU - Duke, V.

AU - Hamblin, T.J.

AU - Wallace, P.

AU - Ely, P.

AU - Clark, E.A.

AU - Lydyard, P.M.

N1 - Note: This work was supported by the Leukaemia Research Fund and INTAS EU [grant number 01-2239].

PY - 2005/11

Y1 - 2005/11

N2 - We have studied the surface expression of the Toll-like receptor family member CD 180 on cells from 78 patients with B-chronic lymphocytic leukaemia (B-CLL). B-CLL cells had variable levels of CD 180 expression, but this was always less than that expressed by normal blood B cells and was stable for 24 months. Significantly higher levels of CD 180 were expressed by B-CLL cells with mutated IGVH genes compared with those using unmutated IGVH genes. This was in contrast to the higher levels of expression of surface immunoglobulin M by B-CLL cells using unmutated, rather than mutated IGVH genes. CD 180 was functional on B-CLL cells from some of the patients, as shown by the increased expression of CD 86 following incubation in vitro with anti-CD 180. The differential expression of CD 180 amongst B-CLL patients is one more marker that may define more precisely the different biological properties of this heterogeneous disease.

AB - We have studied the surface expression of the Toll-like receptor family member CD 180 on cells from 78 patients with B-chronic lymphocytic leukaemia (B-CLL). B-CLL cells had variable levels of CD 180 expression, but this was always less than that expressed by normal blood B cells and was stable for 24 months. Significantly higher levels of CD 180 were expressed by B-CLL cells with mutated IGVH genes compared with those using unmutated IGVH genes. This was in contrast to the higher levels of expression of surface immunoglobulin M by B-CLL cells using unmutated, rather than mutated IGVH genes. CD 180 was functional on B-CLL cells from some of the patients, as shown by the increased expression of CD 86 following incubation in vitro with anti-CD 180. The differential expression of CD 180 amongst B-CLL patients is one more marker that may define more precisely the different biological properties of this heterogeneous disease.

KW - Cancer studies

UR - http://www.ncbi.nlm.nih.gov/pubmed/16225650

U2 - 10.1111/j.1365-2141.2005.05775.x

DO - 10.1111/j.1365-2141.2005.05775.x

M3 - Article

C2 - 16225650

SN - 0007-1048

VL - 131

SP - 313

EP - 319

JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 3

ER -

Differential expression of CD180 and IgM by B-cell chronic lymphocytic leukaemia cells using mutated and unmutated immunoglobulin VH genes

Abstract

Bibliographical note

Keywords

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