Digalactoside expression in the lipopolysaccharide of ' Haemophilus influenzae ' and its role in intravascular survival

Chris D. Bayliss; Ruth Griffin; Mark A. Herbert; Andrew D. Cox; Katherine Makepeace; James C. Richards; Derek W. Hood; E. Richard Moxon

doi:10.1128/IAI.73.10.7022-7026.2005

Digalactoside expression in the lipopolysaccharide of ' Haemophilus influenzae ' and its role in intravascular survival

Chris D. Bayliss, Ruth Griffin, Mark A. Herbert, Andrew D. Cox, Katherine Makepeace, James C. Richards, Derek W. Hood, E. Richard Moxon

Research output: Contribution to journal › Article › peer-review

Abstract

Digalactoside (galα-1-4 galβ) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

Original language	English
Pages (from-to)	7022-7026
Journal	Infection and Immunity
Volume	73
Issue number	10
DOIs	https://doi.org/10.1128/IAI.73.10.7022-7026.2005
Publication status	Published - Oct 2005
Externally published	Yes

Bibliographical note

Note: This work was supported by the Medical Research Council.

Keywords

Biological sciences

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10.1128/IAI.73.10.7022-7026.2005

Griffin-R-33835-VoRFinal published version, 169 KBLicence: Unspecified

http://dx.doi.org/10.1128/IAI.73.10.7022-7026.2005

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title = "Digalactoside expression in the lipopolysaccharide of ' Haemophilus influenzae ' and its role in intravascular survival",

abstract = "Digalactoside (galα-1-4 galβ) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.",

keywords = "Biological sciences",

author = "Bayliss, \{Chris D.\} and Ruth Griffin and Herbert, \{Mark A.\} and Cox, \{Andrew D.\} and Katherine Makepeace and Richards, \{James C.\} and Hood, \{Derek W.\} and Moxon, \{E. Richard\}",

note = "Note: This work was supported by the Medical Research Council.",

year = "2005",

month = oct,

doi = "10.1128/IAI.73.10.7022-7026.2005",

language = "English",

volume = "73",

pages = "7022--7026",

journal = "Infection and Immunity",

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publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Digalactoside expression in the lipopolysaccharide of ' Haemophilus influenzae ' and its role in intravascular survival

AU - Bayliss, Chris D.

AU - Griffin, Ruth

AU - Herbert, Mark A.

AU - Cox, Andrew D.

AU - Makepeace, Katherine

AU - Richards, James C.

AU - Hood, Derek W.

AU - Moxon, E. Richard

N1 - Note: This work was supported by the Medical Research Council.

PY - 2005/10

Y1 - 2005/10

N2 - Digalactoside (galα-1-4 galβ) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

AB - Digalactoside (galα-1-4 galβ) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

KW - Biological sciences

U2 - 10.1128/IAI.73.10.7022-7026.2005

DO - 10.1128/IAI.73.10.7022-7026.2005

M3 - Article

SN - 0019-9567

VL - 73

SP - 7022

EP - 7026

JO - Infection and Immunity

JF - Infection and Immunity

IS - 10

ER -

Digalactoside expression in the lipopolysaccharide of ' Haemophilus influenzae ' and its role in intravascular survival

Abstract

Bibliographical note

Keywords

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