Generation of substance P carbamate in neutral aqueous solution: relevance to inflammatory joint diseases

Geoffrey E. Hawkes; Peter R. Hatcock; Adrian J. Davis; Paul O'Brien; Bruce L. Kidd; Paul I. Mapp; Declan Naughton; Martin Grootveld

doi:10.1016/0014-5793(93)80231-I

Generation of substance P carbamate in neutral aqueous solution: relevance to inflammatory joint diseases

Geoffrey E. Hawkes
, Peter R. Hatcock
, Adrian J. Davis
, Paul O'Brien
, Bruce L. Kidd
, Paul I. Mapp
, Declan Naughton
, Martin Grootveld

Research output: Contribution to journal › Article › peer-review

Abstract

High-field proton (1H) nuclear magnetic resonance (NMR) spectroscopy has been employed to evaluate the formation of substance P carbamate in aqueous solution. Equilibration of substance P with physiologically relevant concentrations of bicarbonate (2.50 x 10(-2) mol.dm-3) at pH 7.00 generated a new multiplet signal centred at 4.13 ppm in its NMR spectrum, characteristic of the alpha-proton of peptide carbamate species. High-field 1H NMR spectroscopy also demonstrated that the model dipeptide, Arg-Gly, formed a carbamate in neutral aqueous solutions containing 2.50 x 10(-2) mol.dm-3 HCO3-. The physiological significance of these results is discussed in view of the central roles of vasoactive neuropeptides in human joint diseases and the hypercapnic environment of the inflamed rheumatoid joint.

Original language	English
Pages (from-to)	249-252
Journal	FEBS Letters
Volume	329
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)80231-I
Publication status	Published - 30 Aug 1993

Bibliographical note

Note: This work was supported by the Arthritis and Rheumatism Council (UK).

Keywords

Allied health professions and studies

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10.1016/0014-5793(93)80231-I

http://dx.doi.org/10.1016/0014-5793(93)80231-I

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title = "Generation of substance P carbamate in neutral aqueous solution: relevance to inflammatory joint diseases",

abstract = "High-field proton (1H) nuclear magnetic resonance (NMR) spectroscopy has been employed to evaluate the formation of substance P carbamate in aqueous solution. Equilibration of substance P with physiologically relevant concentrations of bicarbonate (2.50 x 10(-2) mol.dm-3) at pH 7.00 generated a new multiplet signal centred at 4.13 ppm in its NMR spectrum, characteristic of the alpha-proton of peptide carbamate species. High-field 1H NMR spectroscopy also demonstrated that the model dipeptide, Arg-Gly, formed a carbamate in neutral aqueous solutions containing 2.50 x 10(-2) mol.dm-3 HCO3-. The physiological significance of these results is discussed in view of the central roles of vasoactive neuropeptides in human joint diseases and the hypercapnic environment of the inflamed rheumatoid joint.",

keywords = "Allied health professions and studies",

author = "Hawkes, \{Geoffrey E.\} and Hatcock, \{Peter R.\} and Davis, \{Adrian J.\} and Paul O'Brien and Kidd, \{Bruce L.\} and Mapp, \{Paul I.\} and Declan Naughton and Martin Grootveld",

note = "Note: This work was supported by the Arthritis and Rheumatism Council (UK).",

year = "1993",

month = aug,

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doi = "10.1016/0014-5793(93)80231-I",

language = "English",

volume = "329",

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journal = "FEBS Letters",

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TY - JOUR

T1 - Generation of substance P carbamate in neutral aqueous solution

T2 - relevance to inflammatory joint diseases

AU - Hawkes, Geoffrey E.

AU - Hatcock, Peter R.

AU - Davis, Adrian J.

AU - O'Brien, Paul

AU - Kidd, Bruce L.

AU - Mapp, Paul I.

AU - Naughton, Declan

AU - Grootveld, Martin

N1 - Note: This work was supported by the Arthritis and Rheumatism Council (UK).

PY - 1993/8/30

Y1 - 1993/8/30

N2 - High-field proton (1H) nuclear magnetic resonance (NMR) spectroscopy has been employed to evaluate the formation of substance P carbamate in aqueous solution. Equilibration of substance P with physiologically relevant concentrations of bicarbonate (2.50 x 10(-2) mol.dm-3) at pH 7.00 generated a new multiplet signal centred at 4.13 ppm in its NMR spectrum, characteristic of the alpha-proton of peptide carbamate species. High-field 1H NMR spectroscopy also demonstrated that the model dipeptide, Arg-Gly, formed a carbamate in neutral aqueous solutions containing 2.50 x 10(-2) mol.dm-3 HCO3-. The physiological significance of these results is discussed in view of the central roles of vasoactive neuropeptides in human joint diseases and the hypercapnic environment of the inflamed rheumatoid joint.

AB - High-field proton (1H) nuclear magnetic resonance (NMR) spectroscopy has been employed to evaluate the formation of substance P carbamate in aqueous solution. Equilibration of substance P with physiologically relevant concentrations of bicarbonate (2.50 x 10(-2) mol.dm-3) at pH 7.00 generated a new multiplet signal centred at 4.13 ppm in its NMR spectrum, characteristic of the alpha-proton of peptide carbamate species. High-field 1H NMR spectroscopy also demonstrated that the model dipeptide, Arg-Gly, formed a carbamate in neutral aqueous solutions containing 2.50 x 10(-2) mol.dm-3 HCO3-. The physiological significance of these results is discussed in view of the central roles of vasoactive neuropeptides in human joint diseases and the hypercapnic environment of the inflamed rheumatoid joint.

KW - Allied health professions and studies

UR - http://www.ncbi.nlm.nih.gov/pubmed/7689976

U2 - 10.1016/0014-5793(93)80231-I

DO - 10.1016/0014-5793(93)80231-I

M3 - Article

C2 - 7689976

SN - 0014-5793

VL - 329

SP - 249

EP - 252

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Generation of substance P carbamate in neutral aqueous solution: relevance to inflammatory joint diseases

Abstract

Bibliographical note

Keywords

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