Immunochemical methods for the peptidomic analysis of tachykinin peptides and their precursors

Nigel M. Page; Nicola J. Weston-Bell

doi:10.1007/978-1-60761-535-4

Immunochemical methods for the peptidomic analysis of tachykinin peptides and their precursors

Nigel M. Page, Nicola J. Weston-Bell

Kingston University

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The tachykinins represent the largest known peptide family and are responsible for a range of pleiotropic functions in both vertebrates and invertebrates. Recent research has shown a diversity of mechanisms such as mRNA splicing, precursor processing and post-translation modification that can lead to a complex and continually expanding repertoire of tachykinin peptides. The peptidomic analysis of the tachykinins has been hindered by the lack of specific methodologies to capture, purify and characterise each tachykinin. This chapter summarises some of the methods that have been developed in order to further purify and characterise individual groups of tachykinin peptides from the peptidome.

Original language	English
Title of host publication	Peptidomics
Subtitle of host publication	methods and protocols
Editors	Mikhail Soloviev
Place of Publication	New York, U.S.
Publisher	Humana Press
Pages	293-312
ISBN (Print)	9781607615354
DOIs	https://doi.org/10.1007/978-1-60761-535-4
Publication status	Published - 2010

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Number	615
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Chemistry

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10.1007/978-1-60761-535-4

http://dx.doi.org/10.1007/978-1-60761-535-4

Cite this

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abstract = "The tachykinins represent the largest known peptide family and are responsible for a range of pleiotropic functions in both vertebrates and invertebrates. Recent research has shown a diversity of mechanisms such as mRNA splicing, precursor processing and post-translation modification that can lead to a complex and continually expanding repertoire of tachykinin peptides. The peptidomic analysis of the tachykinins has been hindered by the lack of specific methodologies to capture, purify and characterise each tachykinin. This chapter summarises some of the methods that have been developed in order to further purify and characterise individual groups of tachykinin peptides from the peptidome.",

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Immunochemical methods for the peptidomic analysis of tachykinin peptides and their precursors. / Page, Nigel M.; Weston-Bell, Nicola J.
Peptidomics: methods and protocols. ed. / Mikhail Soloviev. New York, U.S.: Humana Press, 2010. p. 293-312 (Methods in Molecular Biology; No. 615).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Weston-Bell, Nicola J.

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N2 - The tachykinins represent the largest known peptide family and are responsible for a range of pleiotropic functions in both vertebrates and invertebrates. Recent research has shown a diversity of mechanisms such as mRNA splicing, precursor processing and post-translation modification that can lead to a complex and continually expanding repertoire of tachykinin peptides. The peptidomic analysis of the tachykinins has been hindered by the lack of specific methodologies to capture, purify and characterise each tachykinin. This chapter summarises some of the methods that have been developed in order to further purify and characterise individual groups of tachykinin peptides from the peptidome.

AB - The tachykinins represent the largest known peptide family and are responsible for a range of pleiotropic functions in both vertebrates and invertebrates. Recent research has shown a diversity of mechanisms such as mRNA splicing, precursor processing and post-translation modification that can lead to a complex and continually expanding repertoire of tachykinin peptides. The peptidomic analysis of the tachykinins has been hindered by the lack of specific methodologies to capture, purify and characterise each tachykinin. This chapter summarises some of the methods that have been developed in order to further purify and characterise individual groups of tachykinin peptides from the peptidome.

KW - Chemistry

UR - http://www.ncbi.nlm.nih.gov/pubmed/20013217

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PB - Humana Press

CY - New York, U.S.

ER -

Immunochemical methods for the peptidomic analysis of tachykinin peptides and their precursors

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