Immunoproteomic characterisation of Mycoplasma mycoides subspecies capri by mass spectrometry analysis of two-dimensional electrophoresis spots and western blot.

Colin P. Churchward; Ruben S Rosales; Adriana Gielbert; Mercedes Dom├¡nguez; Robin A J Nicholas; Roger D Ayling

doi:10.1111/jphp.12344

Immunoproteomic characterisation of Mycoplasma mycoides subspecies capri by mass spectrometry analysis of two-dimensional electrophoresis spots and western blot.

Colin P. Churchward, Ruben S Rosales, Adriana Gielbert, Mercedes Dom├¡nguez, Robin A J Nicholas, Roger D Ayling

Research output: Contribution to journal › Article › peer-review

Abstract

Six proteins were found to have a less than 95% amino acid similarity to a closely related Mycoplasma species showing that they warrant further evaluation in development of diagnostic tests. These proteins were a dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex, phosphoglycerate kinase, pyrimidine-nucleoside phosphorylase, 30S ribosomal protein S6, ribulose-phosphate 3-epimerase and D-lactate dehydrogenase.

Original language	English
Pages (from-to)	364-374
Journal	Journal of Pharmacy and Pharmacology
Volume	67
Issue number	3
DOIs	https://doi.org/10.1111/jphp.12344
Publication status	Published - Mar 2015
Externally published	Yes

Bibliographical note

Note: This work was supported by the Department for Envrionment, Food and Rural Affairs (DEFRA) grant number SE0798.

Keywords

biological characterisation of novel molecules
immunoproteomics
Pharmacy

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10.1111/jphp.12344

http://dx.doi.org/10.1111/jphp.12344

Cite this

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title = "Immunoproteomic characterisation of Mycoplasma mycoides subspecies capri by mass spectrometry analysis of two-dimensional electrophoresis spots and western blot.",

abstract = "Six proteins were found to have a less than 95\% amino acid similarity to a closely related Mycoplasma species showing that they warrant further evaluation in development of diagnostic tests. These proteins were a dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex, phosphoglycerate kinase, pyrimidine-nucleoside phosphorylase, 30S ribosomal protein S6, ribulose-phosphate 3-epimerase and D-lactate dehydrogenase.",

keywords = "biological characterisation of novel molecules, immunoproteomics, Pharmacy",

author = "Churchward, \{Colin P.\} and Rosales, \{Ruben S\} and Adriana Gielbert and Mercedes Dom├¡nguez and Nicholas, \{Robin A J\} and Ayling, \{Roger D\}",

note = "Note: This work was supported by the Department for Envrionment, Food and Rural Affairs (DEFRA) grant number SE0798.",

year = "2015",

month = mar,

doi = "10.1111/jphp.12344",

language = "English",

volume = "67",

pages = "364--374",

journal = "Journal of Pharmacy and Pharmacology",

issn = "0022-3573",

publisher = "Oxford University Press",

number = "3",

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Immunoproteomic characterisation of Mycoplasma mycoides subspecies capri by mass spectrometry analysis of two-dimensional electrophoresis spots and western blot. / Churchward, Colin P.; Rosales, Ruben S; Gielbert, Adriana et al.
In: Journal of Pharmacy and Pharmacology, Vol. 67, No. 3, 03.2015, p. 364-374.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Immunoproteomic characterisation of Mycoplasma mycoides subspecies capri by mass spectrometry analysis of two-dimensional electrophoresis spots and western blot.

AU - Churchward, Colin P.

AU - Rosales, Ruben S

AU - Gielbert, Adriana

AU - Dom├¡nguez, Mercedes

AU - Nicholas, Robin A J

AU - Ayling, Roger D

N1 - Note: This work was supported by the Department for Envrionment, Food and Rural Affairs (DEFRA) grant number SE0798.

PY - 2015/3

Y1 - 2015/3

N2 - Six proteins were found to have a less than 95% amino acid similarity to a closely related Mycoplasma species showing that they warrant further evaluation in development of diagnostic tests. These proteins were a dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex, phosphoglycerate kinase, pyrimidine-nucleoside phosphorylase, 30S ribosomal protein S6, ribulose-phosphate 3-epimerase and D-lactate dehydrogenase.

AB - Six proteins were found to have a less than 95% amino acid similarity to a closely related Mycoplasma species showing that they warrant further evaluation in development of diagnostic tests. These proteins were a dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex, phosphoglycerate kinase, pyrimidine-nucleoside phosphorylase, 30S ribosomal protein S6, ribulose-phosphate 3-epimerase and D-lactate dehydrogenase.

KW - biological characterisation of novel molecules

KW - immunoproteomics

KW - Pharmacy

U2 - 10.1111/jphp.12344

DO - 10.1111/jphp.12344

M3 - Article

C2 - 25495903

SN - 0022-3573

VL - 67

SP - 364

EP - 374

JO - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

IS - 3

ER -

Immunoproteomic characterisation of Mycoplasma mycoides subspecies capri by mass spectrometry analysis of two-dimensional electrophoresis spots and western blot.

Abstract

Bibliographical note

Keywords

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