Insect infection model for Campylobacter jejuni reveals that O-methyl phosphoramidate has insecticidal activity

Nicola J Senior; Martin Woodward; Olivia L Champion; Andrey V Karlyshev; Roberto La Ragione; Sarah L Howard; Brendan W Wren; Richard W Titball

doi:10.1086/650494

Insect infection model for Campylobacter jejuni reveals that O-methyl phosphoramidate has insecticidal activity

Nicola J Senior
, Martin Woodward
, Olivia L Champion
, Andrey V Karlyshev
, Roberto La Ragione
, Sarah L Howard
, Brendan W Wren
, Richard W Titball

Research output: Contribution to journal › Article › peer-review

Abstract

Galleria mellonella (wax moth) larvae have elsewhere been shown to be susceptible to pathogens such as Francisella tularensis, Burkholderia mallei, and Pseudomonas aeruginosa. We report that the larvae are rapidly killed by Campylobacter jejuni at 37 degrees C. Three strains of C. jejuni tested, 11168H (human diarrheal isolate), G1 (human Guillain-Barré syndrome isolate), and 81-176 (human diarrheal isolate), were equally effective at killing G. mellonella larvae. A panel of defined mutants of C. jejuni 11168H, in known or putative virulence genes, showed different degrees of attenuation in G. mellonella larvae. A mutant lacking the O-methyl phosphoramidate (MeOPN) capsule side group was attenuated, clearly demonstrating that MeOPN has a role in virulence. This new model of C. jejuni infection should facilitate the identification of novel virulence genes.

Original language	English
Pages (from-to)	776-782
Journal	The Journal of Infectious Diseases
Volume	201
Issue number	5
DOIs	https://doi.org/10.1086/650494
Publication status	Published - Mar 2010

Keywords

Biological sciences

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http://dx.doi.org/10.1086/650494

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title = "Insect infection model for Campylobacter jejuni reveals that O-methyl phosphoramidate has insecticidal activity",

abstract = "Galleria mellonella (wax moth) larvae have elsewhere been shown to be susceptible to pathogens such as Francisella tularensis, Burkholderia mallei, and Pseudomonas aeruginosa. We report that the larvae are rapidly killed by Campylobacter jejuni at 37 degrees C. Three strains of C. jejuni tested, 11168H (human diarrheal isolate), G1 (human Guillain-Barr{\'e} syndrome isolate), and 81-176 (human diarrheal isolate), were equally effective at killing G. mellonella larvae. A panel of defined mutants of C. jejuni 11168H, in known or putative virulence genes, showed different degrees of attenuation in G. mellonella larvae. A mutant lacking the O-methyl phosphoramidate (MeOPN) capsule side group was attenuated, clearly demonstrating that MeOPN has a role in virulence. This new model of C. jejuni infection should facilitate the identification of novel virulence genes.",

keywords = "Biological sciences",

author = "Senior, \{Nicola J\} and Martin Woodward and Champion, \{Olivia L\} and Karlyshev, \{Andrey V\} and \{La Ragione\}, Roberto and Howard, \{Sarah L\} and Wren, \{Brendan W\} and Titball, \{Richard W\}",

year = "2010",

month = mar,

doi = "10.1086/650494",

language = "English",

volume = "201",

pages = "776--782",

journal = "The Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "5",

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TY - JOUR

T1 - Insect infection model for Campylobacter jejuni reveals that O-methyl phosphoramidate has insecticidal activity

AU - Senior, Nicola J

AU - Woodward, Martin

AU - Champion, Olivia L

AU - Karlyshev, Andrey V

AU - La Ragione, Roberto

AU - Howard, Sarah L

AU - Wren, Brendan W

AU - Titball, Richard W

PY - 2010/3

Y1 - 2010/3

N2 - Galleria mellonella (wax moth) larvae have elsewhere been shown to be susceptible to pathogens such as Francisella tularensis, Burkholderia mallei, and Pseudomonas aeruginosa. We report that the larvae are rapidly killed by Campylobacter jejuni at 37 degrees C. Three strains of C. jejuni tested, 11168H (human diarrheal isolate), G1 (human Guillain-Barré syndrome isolate), and 81-176 (human diarrheal isolate), were equally effective at killing G. mellonella larvae. A panel of defined mutants of C. jejuni 11168H, in known or putative virulence genes, showed different degrees of attenuation in G. mellonella larvae. A mutant lacking the O-methyl phosphoramidate (MeOPN) capsule side group was attenuated, clearly demonstrating that MeOPN has a role in virulence. This new model of C. jejuni infection should facilitate the identification of novel virulence genes.

AB - Galleria mellonella (wax moth) larvae have elsewhere been shown to be susceptible to pathogens such as Francisella tularensis, Burkholderia mallei, and Pseudomonas aeruginosa. We report that the larvae are rapidly killed by Campylobacter jejuni at 37 degrees C. Three strains of C. jejuni tested, 11168H (human diarrheal isolate), G1 (human Guillain-Barré syndrome isolate), and 81-176 (human diarrheal isolate), were equally effective at killing G. mellonella larvae. A panel of defined mutants of C. jejuni 11168H, in known or putative virulence genes, showed different degrees of attenuation in G. mellonella larvae. A mutant lacking the O-methyl phosphoramidate (MeOPN) capsule side group was attenuated, clearly demonstrating that MeOPN has a role in virulence. This new model of C. jejuni infection should facilitate the identification of novel virulence genes.

KW - Biological sciences

UR - http://www.ncbi.nlm.nih.gov/pubmed/20113177

U2 - 10.1086/650494

DO - 10.1086/650494

M3 - Article

C2 - 20113177

SN - 0022-1899

VL - 201

SP - 776

EP - 782

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

IS - 5

ER -

Insect infection model for Campylobacter jejuni reveals that O-methyl phosphoramidate has insecticidal activity

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Keywords

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