Label-free detection of transgenic crops using an isothermal amplification reporting CRISPR/Cas12 assay

Xiaoying Zhu; Hao Yang; Mian Wang; Minghua Wu; Mohammad Rizwan Khan; Aimin Luo; Sha Deng; Rosa Busquets; Guiping He; Ruijie Deng

doi:10.1021/acssynbio.1c00428

Label-free detection of transgenic crops using an isothermal amplification reporting CRISPR/Cas12 assay

Xiaoying Zhu
, Hao Yang
, Mian Wang
, Minghua Wu
, Mohammad Rizwan Khan
, Aimin Luo
, Sha Deng
, Rosa Busquets
, Guiping He
, Ruijie Deng

Research output: Contribution to journal › Article › peer-review

6 Downloads (Pure)

Original language	English
Pages (from-to)	317-324
Journal	ACS Synthetic Biology
Volume	11
Issue number	1
Early online date	17 Dec 2021
DOIs	https://doi.org/10.1021/acssynbio.1c00428
Publication status	Published - 21 Jan 2022

Bibliographical note

Note: This work was supported by the National Natural Science
Foundation of China (No. 22074100), the Young Elite
Scientist Sponsorship Program by CAST (No.
YESS20200036), the SichuanÔêÆLuzhou Strategic Cooperation
Project (No. 2019CDLZ-02), the SichuanÔêÆLuzhou Strategic
Cooperation Project (No. 2020CDLZ-3-SCU), the Sichuan
Technological Transformation Program (No.
2019ZHCG0073), and the Researchers Supporting Project
Number RSP-2021/138, King Saud University, Riyadh, Saudi
Arabia.

Keywords

Agriculture, veterinary and food science
CRISPR/Cas12
G-quadruplex
food authenticity
isothermal amplification
transgenic crops

Access to Document

10.1021/acssynbio.1c00428

Busquets-R-50898-AAMAccepted author manuscript, 1.03 MBLicence: Unspecified

https://doi.org/10.1021/acssynbio.1c00428

Cite this

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title = "Label-free detection of transgenic crops using an isothermal amplification reporting CRISPR/Cas12 assay",

keywords = "Agriculture, veterinary and food science, CRISPR/Cas12, G-quadruplex, food authenticity, isothermal amplification, transgenic crops",

author = "Xiaoying Zhu and Hao Yang and Mian Wang and Minghua Wu and Khan, \{Mohammad Rizwan\} and Aimin Luo and Sha Deng and Rosa Busquets and Guiping He and Ruijie Deng",

note = "Note: This work was supported by the National Natural Science Foundation of China (No. 22074100), the Young Elite Scientist Sponsorship Program by CAST (No. YESS20200036), the Sichuan{\^O}{\^e}{\AE}Luzhou Strategic Cooperation Project (No. 2019CDLZ-02), the Sichuan{\^O}{\^e}{\AE}Luzhou Strategic Cooperation Project (No. 2020CDLZ-3-SCU), the Sichuan Technological Transformation Program (No. 2019ZHCG0073), and the Researchers Supporting Project Number RSP-2021/138, King Saud University, Riyadh, Saudi Arabia.",

year = "2022",

month = jan,

day = "21",

doi = "10.1021/acssynbio.1c00428",

language = "English",

volume = "11",

pages = "317--324",

journal = "ACS Synthetic Biology",

issn = "2161-5063",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Label-free detection of transgenic crops using an isothermal amplification reporting CRISPR/Cas12 assay

AU - Zhu, Xiaoying

AU - Yang, Hao

AU - Wang, Mian

AU - Wu, Minghua

AU - Khan, Mohammad Rizwan

AU - Luo, Aimin

AU - Deng, Sha

AU - Busquets, Rosa

AU - He, Guiping

AU - Deng, Ruijie

N1 - Note: This work was supported by the National Natural Science Foundation of China (No. 22074100), the Young Elite Scientist Sponsorship Program by CAST (No. YESS20200036), the SichuanÔêÆLuzhou Strategic Cooperation Project (No. 2019CDLZ-02), the SichuanÔêÆLuzhou Strategic Cooperation Project (No. 2020CDLZ-3-SCU), the Sichuan Technological Transformation Program (No. 2019ZHCG0073), and the Researchers Supporting Project Number RSP-2021/138, King Saud University, Riyadh, Saudi Arabia.

PY - 2022/1/21

Y1 - 2022/1/21

KW - Agriculture, veterinary and food science

KW - CRISPR/Cas12

KW - G-quadruplex

KW - food authenticity

KW - isothermal amplification

KW - transgenic crops

U2 - 10.1021/acssynbio.1c00428

DO - 10.1021/acssynbio.1c00428

M3 - Article

SN - 2161-5063

VL - 11

SP - 317

EP - 324

JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

IS - 1

ER -