TY - JOUR
T1 - Mitogenic activity of insulin in the culture of a trypanosome
T2 - duration and dose response
AU - Mackintosh, David
AU - Coleman, Kim
AU - Davies, Angela J.
PY - 2003/1
Y1 - 2003/1
N2 - The effects of insulin on the in vitro growth characteristics of Trypanosoma granulosum were investigated. The insulin growth stimulus had a rapid onset with little or no lag phase but was relatively short lived, growth peaking typically on day 3 or 4 of culture. This contrasted with medium containing 10% foetal calf serum, normally used for in vitro culture of this trypanosome, which after a 3 day lag stimulated sustained log-phase growth. Insulin demonstrated a biphasic dose response with maximum growth typically occurring at 3-10 microg/ml and a much reduced effect at higher concentrations. These findings suggest a possible role for host insulin and/or trypanosomal insulin-like molecules in regulating parasite growth in vivo.
AB - The effects of insulin on the in vitro growth characteristics of Trypanosoma granulosum were investigated. The insulin growth stimulus had a rapid onset with little or no lag phase but was relatively short lived, growth peaking typically on day 3 or 4 of culture. This contrasted with medium containing 10% foetal calf serum, normally used for in vitro culture of this trypanosome, which after a 3 day lag stimulated sustained log-phase growth. Insulin demonstrated a biphasic dose response with maximum growth typically occurring at 3-10 microg/ml and a much reduced effect at higher concentrations. These findings suggest a possible role for host insulin and/or trypanosomal insulin-like molecules in regulating parasite growth in vivo.
KW - Allied health professions and studies
UR - http://www.ncbi.nlm.nih.gov/pubmed/12713803
U2 - 10.1016/S1065-6995(02)00251-2
DO - 10.1016/S1065-6995(02)00251-2
M3 - Article
C2 - 12713803
SN - 1065-6995
VL - 27
SP - 75
EP - 78
JO - Cell Biology International
JF - Cell Biology International
IS - 1
ER -