TY - JOUR
T1 - Molecular phylogenetics of tsetse flies (Diptera
T2 - Glossinidae) based on mitochondrial (COI, 16S, ND2) and nuclear ribosomal DNA sequences, with an emphasis on the palpalis group
AU - Solano, P.
AU - Donnelly, M.J
AU - Dyer, N.A
AU - Lawton, S.P.
AU - Ravel, S.
AU - Choi, K.S
AU - Lehane, M.J
AU - Robinson, A.S
AU - Okedi, L.M
AU - Hall, M.J
PY - 2008/10
Y1 - 2008/10
N2 - Relationships of 13 species of the genus Glossina (tsetse flies) were inferred from mitochondrial (cytochrome oxidase 1, NADH dehydrogenase 2 and 16S) and nuclear (internal transcribed spacer 1 of rDNA) sequences. The resulting phylogeny confirms the monophyly of the morphologically defined fusca, morsitans and palpalis subgenera. Genetic distances between palpalis and morsitans subspecies suggest that their status needs revision. In particular, cytochrome oxidase 1 sequences showed large geographical differences within G. palpalis palpalis, suggesting the existence of cryptic species within this subspecies. The morphology of palpalis group female genital plates was examined, and individuals were found varying outside the ranges specified by the standard identification keys, making definitive morphological classification impossible. A diagnostic PCR to distinguish G. palpalis palpalis, G. tachinoides and G. palpalis gambiensis based on length differences of internal transcribed spacer 1 sequences is presented
AB - Relationships of 13 species of the genus Glossina (tsetse flies) were inferred from mitochondrial (cytochrome oxidase 1, NADH dehydrogenase 2 and 16S) and nuclear (internal transcribed spacer 1 of rDNA) sequences. The resulting phylogeny confirms the monophyly of the morphologically defined fusca, morsitans and palpalis subgenera. Genetic distances between palpalis and morsitans subspecies suggest that their status needs revision. In particular, cytochrome oxidase 1 sequences showed large geographical differences within G. palpalis palpalis, suggesting the existence of cryptic species within this subspecies. The morphology of palpalis group female genital plates was examined, and individuals were found varying outside the ranges specified by the standard identification keys, making definitive morphological classification impossible. A diagnostic PCR to distinguish G. palpalis palpalis, G. tachinoides and G. palpalis gambiensis based on length differences of internal transcribed spacer 1 sequences is presented
KW - Agriculture, veterinary and food science
UR - https://www.ncbi.nlm.nih.gov/pubmed/18692147
U2 - 10.1016/j.ympev.2008.07.011
DO - 10.1016/j.ympev.2008.07.011
M3 - Article
C2 - 18692147
SN - 1055-7903
VL - 49
SP - 227
EP - 239
JO - Molecular Phylogenetics and Evolution
JF - Molecular Phylogenetics and Evolution
ER -