Phagocytosis by Lymnaea stagnalis haemocytes: a potential role for phosphatidylinositol 3-kinase but not protein kinase A

Louise D. Plows; Richard T. Cook; Angela J. Davies; Anthony J. Walker

doi:10.1016/j.jip.2005.10.011

Phagocytosis by Lymnaea stagnalis haemocytes: a potential role for phosphatidylinositol 3-kinase but not protein kinase A

Louise D. Plows
, Richard T. Cook
, Angela J. Davies
, Anthony J. Walker

Research output: Contribution to journal › Article › peer-review

Abstract

The molecular events that regulate phagocytosis, an important innate immune response, in invertebrate defence cells (haemocytes) are poorly understood. Lymnaea stagnalis haemocytes were used as a model to elucidate the role of cell signalling pathways in phagocytosis by molluscan defence cells. The phosphatidylinositol 3-kinase (PI3-K) inhibitor, LY294002, significantly impaired haemocyte phagocytic activity in a dose-responsive manner with 10 microM LY294002 reducing internalization of fluorescent-conjugated Escherichia coli by 62% (P < or = 0.001). In contrast, the protein kinase A (PKA) inhibitor KT5720 was without effect. Therefore, PI3-K, but not PKA, appears to control phagocytosis by haemocytes in these gastropod molluscs.

Original language	English
Pages (from-to)	74-77
Journal	Journal of Invertebrate Pathology
Volume	91
Issue number	1
DOIs	https://doi.org/10.1016/j.jip.2005.10.011
Publication status	Published - Jan 2006

Bibliographical note

Note: This work was supported by the Royal Society and Kingston University

Keywords

Biological sciences

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10.1016/j.jip.2005.10.011

http://dx.doi.org/10.1016/j.jip.2005.10.011

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title = "Phagocytosis by Lymnaea stagnalis haemocytes: a potential role for phosphatidylinositol 3-kinase but not protein kinase A",

abstract = "The molecular events that regulate phagocytosis, an important innate immune response, in invertebrate defence cells (haemocytes) are poorly understood. Lymnaea stagnalis haemocytes were used as a model to elucidate the role of cell signalling pathways in phagocytosis by molluscan defence cells. The phosphatidylinositol 3-kinase (PI3-K) inhibitor, LY294002, significantly impaired haemocyte phagocytic activity in a dose-responsive manner with 10 microM LY294002 reducing internalization of fluorescent-conjugated Escherichia coli by 62\% (P < or = 0.001). In contrast, the protein kinase A (PKA) inhibitor KT5720 was without effect. Therefore, PI3-K, but not PKA, appears to control phagocytosis by haemocytes in these gastropod molluscs.",

keywords = "Biological sciences",

author = "Plows, \{Louise D.\} and Cook, \{Richard T.\} and Davies, \{Angela J.\} and Walker, \{Anthony J.\}",

note = "Note: This work was supported by the Royal Society and Kingston University",

year = "2006",

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doi = "10.1016/j.jip.2005.10.011",

language = "English",

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T2 - a potential role for phosphatidylinositol 3-kinase but not protein kinase A

AU - Plows, Louise D.

AU - Cook, Richard T.

AU - Davies, Angela J.

AU - Walker, Anthony J.

N1 - Note: This work was supported by the Royal Society and Kingston University

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AB - The molecular events that regulate phagocytosis, an important innate immune response, in invertebrate defence cells (haemocytes) are poorly understood. Lymnaea stagnalis haemocytes were used as a model to elucidate the role of cell signalling pathways in phagocytosis by molluscan defence cells. The phosphatidylinositol 3-kinase (PI3-K) inhibitor, LY294002, significantly impaired haemocyte phagocytic activity in a dose-responsive manner with 10 microM LY294002 reducing internalization of fluorescent-conjugated Escherichia coli by 62% (P < or = 0.001). In contrast, the protein kinase A (PKA) inhibitor KT5720 was without effect. Therefore, PI3-K, but not PKA, appears to control phagocytosis by haemocytes in these gastropod molluscs.

KW - Biological sciences

UR - http://www.ncbi.nlm.nih.gov/pubmed/16376929

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DO - 10.1016/j.jip.2005.10.011

M3 - Article

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VL - 91

SP - 74

EP - 77

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

IS - 1

ER -

Phagocytosis by Lymnaea stagnalis haemocytes: a potential role for phosphatidylinositol 3-kinase but not protein kinase A

Abstract

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