Ratiometric G-quadruplex assay for robust lead detection in
food samples

Yumei Liu; Hao Yang; Rui Wan; Mohammad Rizwan Khan; Nan Wang; Rosa Busquets; Ruijie Deng; Qiang He; Zhifeng Zhao

doi:10.3390/bios11080274

Ratiometric G-quadruplex assay for robust lead detection in food samples

Yumei Liu
, Hao Yang
, Rui Wan
, Mohammad Rizwan Khan
, Nan Wang
, Rosa Busquets
, Ruijie Deng
, Qiang He
, Zhifeng Zhao

Research output: Contribution to journal › Article › peer-review

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Abstract

Lead (Pb2+) pollution is a serious food safety issue, rapid detection of Pb2+ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb2+ supervision in food samples. Pb2+ specific aptamer can bolster a transition of G-quadruplex structural response to Pb2+; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb2+, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb2+ detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb2+ testing associated with food safety

Original language	English
Article number	274
Journal	Biosensors
Volume	11
Issue number	8
Early online date	16 Aug 2021
DOIs	https://doi.org/10.3390/bios11080274
Publication status	Published - 31 Aug 2021

Bibliographical note

Note: This work was supported by National Natural Science Foundation of China [grant number: 22074100], the Green Manufacturing Project of Ministry of Industry and Information Technology of China, and the Researchers Supporting Project Number (RSP-2021/138), King Saud University, Riyadh, Saudi Arabia.

Impact: This research improves food safety

Keywords

Chemistry

Access to Document

10.3390/bios11080274

Busquests-R-49844-VoRFinal published version, 2.1 MBLicence: CC BY

https://doi.org/10.3390/bios11080274

Cite this

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title = "Ratiometric G-quadruplex assay for robust lead detection in food samples",

abstract = "Lead (Pb2+) pollution is a serious food safety issue, rapid detection of Pb2+ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb2+ supervision in food samples. Pb2+ specific aptamer can bolster a transition of G-quadruplex structural response to Pb2+; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb2+, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb2+ detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb2+ testing associated with food safety",

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author = "Yumei Liu and Hao Yang and Rui Wan and Khan, \{Mohammad Rizwan\} and Nan Wang and Rosa Busquets and Ruijie Deng and Qiang He and Zhifeng Zhao",

note = "Note: This work was supported by National Natural Science Foundation of China [grant number: 22074100], the Green Manufacturing Project of Ministry of Industry and Information Technology of China, and the Researchers Supporting Project Number (RSP-2021/138), King Saud University, Riyadh, Saudi Arabia. Impact: This research improves food safety",

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T1 - Ratiometric G-quadruplex assay for robust lead detection in food samples

AU - Liu, Yumei

AU - Yang, Hao

AU - Wan, Rui

AU - Khan, Mohammad Rizwan

AU - Wang, Nan

AU - Busquets, Rosa

AU - Deng, Ruijie

AU - He, Qiang

AU - Zhao, Zhifeng

N1 - Note: This work was supported by National Natural Science Foundation of China [grant number: 22074100], the Green Manufacturing Project of Ministry of Industry and Information Technology of China, and the Researchers Supporting Project Number (RSP-2021/138), King Saud University, Riyadh, Saudi Arabia. Impact: This research improves food safety

PY - 2021/8/31

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N2 - Lead (Pb2+) pollution is a serious food safety issue, rapid detection of Pb2+ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb2+ supervision in food samples. Pb2+ specific aptamer can bolster a transition of G-quadruplex structural response to Pb2+; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb2+, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb2+ detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb2+ testing associated with food safety

AB - Lead (Pb2+) pollution is a serious food safety issue, rapid detection of Pb2+ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb2+ supervision in food samples. Pb2+ specific aptamer can bolster a transition of G-quadruplex structural response to Pb2+; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb2+, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb2+ detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb2+ testing associated with food safety

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