Signature-tagged transposon mutagenesis identifies novel ' Mycobacterium tuberculosis ' genes involved in the parasitism of human macrophages

Vania Rosas-Magallanes; Gustavo Stadthagen-Gomez; Jean Rauzier; Luis B. Barreiro; Ludovic Tailleux; Frederic Boudou; Ruth Griffin; Jerome Nigou; Mary Jackson; Brigitte Gicquel; Olivier Neyrolles

Signature-tagged transposon mutagenesis identifies novel ' Mycobacterium tuberculosis ' genes involved in the parasitism of human macrophages

Vania Rosas-Magallanes, Gustavo Stadthagen-Gomez, Jean Rauzier, Luis B. Barreiro, Ludovic Tailleux, Frederic Boudou, Ruth Griffin, Jerome Nigou, Mary Jackson, Brigitte Gicquel, Olivier Neyrolles

Research output: Contribution to journal › Article › peer-review

Abstract

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

Original language	English
Pages (from-to)	504-507
Journal	Infection and Immunity
Volume	75
Issue number	1
Publication status	Published - Jan 2007
Externally published	Yes

Bibliographical note

Note: This work was supported by Institut Pasteur and the European
Community [contracts QLK2-CT-2001-02018 and QLK2-CT-2000-
01761].

Keywords

Biological sciences

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Griffin-R-33824-VoRFinal published version, 210 KBLicence: Unspecified

Cite this

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title = "Signature-tagged transposon mutagenesis identifies novel ' Mycobacterium tuberculosis ' genes involved in the parasitism of human macrophages",

abstract = "Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.",

keywords = "Biological sciences",

author = "Vania Rosas-Magallanes and Gustavo Stadthagen-Gomez and Jean Rauzier and Barreiro, \{Luis B.\} and Ludovic Tailleux and Frederic Boudou and Ruth Griffin and Jerome Nigou and Mary Jackson and Brigitte Gicquel and Olivier Neyrolles",

note = "Note: This work was supported by Institut Pasteur and the European Community [contracts QLK2-CT-2001-02018 and QLK2-CT-2000- 01761].",

year = "2007",

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language = "English",

volume = "75",

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journal = "Infection and Immunity",

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T1 - Signature-tagged transposon mutagenesis identifies novel ' Mycobacterium tuberculosis ' genes involved in the parasitism of human macrophages

AU - Rosas-Magallanes, Vania

AU - Stadthagen-Gomez, Gustavo

AU - Rauzier, Jean

AU - Barreiro, Luis B.

AU - Tailleux, Ludovic

AU - Boudou, Frederic

AU - Griffin, Ruth

AU - Nigou, Jerome

AU - Jackson, Mary

AU - Gicquel, Brigitte

AU - Neyrolles, Olivier

N1 - Note: This work was supported by Institut Pasteur and the European Community [contracts QLK2-CT-2001-02018 and QLK2-CT-2000- 01761].

PY - 2007/1

Y1 - 2007/1

N2 - Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

AB - Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

KW - Biological sciences

UR - https://www.ncbi.nlm.nih.gov/pubmed/17030567

M3 - Article

C2 - 17030567

SN - 0019-9567

VL - 75

SP - 504

EP - 507

JO - Infection and Immunity

JF - Infection and Immunity

IS - 1

ER -

Signature-tagged transposon mutagenesis identifies novel ' Mycobacterium tuberculosis ' genes involved in the parasitism of human macrophages

Abstract

Bibliographical note

Keywords

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