Synthesis and initial evaluation of a novel fluorophore for selective FMDV 3C Protease detection

Samerah Malik; Alex Sinclair; Ali Ryan; Adam Le Gresley

doi:10.3390/molecules25163599

Synthesis and initial evaluation of a novel fluorophore for selective FMDV 3C Protease detection

Samerah Malik, Alex Sinclair, Ali Ryan, Adam Le Gresley

Kingston University

Research output: Contribution to journal › Article › peer-review

Abstract

The development and evaluation of a Boc-AL(Boc)Q(Trt)-AMC fluorophore to detect 3C Protease, produced by Foot and Mouth Disease Virus (FMDV) is reported, with a view to a potential use as a rapid screen for FMDV infected livestock The peptide-linked conjugate fluorophore is evaluated in vitro for sensitivity, specificity, stability and rapidity and shows statistically significant increases in fluorescence when exposed to physiologically relevant concentrations of 3C Protease and selectivity when compared with other common proteases likely to be located, typically in the absence of FMDV. The stability of deprotected Boc-AL(Boc)Q(Trt)-AMC is reported as a limitation of this probe.

Original language	English
Article number	3599
Journal	Molecules
Volume	25
Issue number	16
Early online date	7 Aug 2020
DOIs	https://doi.org/10.3390/molecules25163599
Publication status	Published - 7 Aug 2020

Bibliographical note

Note: This work was supported by Kingston University through a PhD Scholarship.

Keywords

3C protease
Chemistry
FMDV
fluorescence
veterinary testing

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10.3390/molecules25163599

Malik-S-46730-VoRFinal published version, 1.2 MBLicence: CC BY

https://doi.org/10.3390/molecules25163599

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title = "Synthesis and initial evaluation of a novel fluorophore for selective FMDV 3C Protease detection",

abstract = "The development and evaluation of a Boc-AL(Boc)Q(Trt)-AMC fluorophore to detect 3C Protease, produced by Foot and Mouth Disease Virus (FMDV) is reported, with a view to a potential use as a rapid screen for FMDV infected livestock The peptide-linked conjugate fluorophore is evaluated in vitro for sensitivity, specificity, stability and rapidity and shows statistically significant increases in fluorescence when exposed to physiologically relevant concentrations of 3C Protease and selectivity when compared with other common proteases likely to be located, typically in the absence of FMDV. The stability of deprotected Boc-AL(Boc)Q(Trt)-AMC is reported as a limitation of this probe.",

keywords = "3C protease, Chemistry, FMDV, fluorescence, veterinary testing",

author = "Samerah Malik and Alex Sinclair and Ali Ryan and \{Le Gresley\}, Adam",

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doi = "10.3390/molecules25163599",

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T1 - Synthesis and initial evaluation of a novel fluorophore for selective FMDV 3C Protease detection

AU - Malik, Samerah

AU - Sinclair, Alex

AU - Ryan, Ali

AU - Le Gresley, Adam

N1 - Note: This work was supported by Kingston University through a PhD Scholarship.

PY - 2020/8/7

Y1 - 2020/8/7

N2 - The development and evaluation of a Boc-AL(Boc)Q(Trt)-AMC fluorophore to detect 3C Protease, produced by Foot and Mouth Disease Virus (FMDV) is reported, with a view to a potential use as a rapid screen for FMDV infected livestock The peptide-linked conjugate fluorophore is evaluated in vitro for sensitivity, specificity, stability and rapidity and shows statistically significant increases in fluorescence when exposed to physiologically relevant concentrations of 3C Protease and selectivity when compared with other common proteases likely to be located, typically in the absence of FMDV. The stability of deprotected Boc-AL(Boc)Q(Trt)-AMC is reported as a limitation of this probe.

AB - The development and evaluation of a Boc-AL(Boc)Q(Trt)-AMC fluorophore to detect 3C Protease, produced by Foot and Mouth Disease Virus (FMDV) is reported, with a view to a potential use as a rapid screen for FMDV infected livestock The peptide-linked conjugate fluorophore is evaluated in vitro for sensitivity, specificity, stability and rapidity and shows statistically significant increases in fluorescence when exposed to physiologically relevant concentrations of 3C Protease and selectivity when compared with other common proteases likely to be located, typically in the absence of FMDV. The stability of deprotected Boc-AL(Boc)Q(Trt)-AMC is reported as a limitation of this probe.

KW - 3C protease

KW - Chemistry

KW - FMDV

KW - fluorescence

KW - veterinary testing

U2 - 10.3390/molecules25163599

DO - 10.3390/molecules25163599

M3 - Article

SN - 1420-3049

VL - 25

JO - Molecules

JF - Molecules

IS - 16

M1 - 3599

ER -

Synthesis and initial evaluation of a novel fluorophore for selective FMDV 3C Protease detection

Abstract

Bibliographical note

Keywords

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