Abstract
Mercury is a threatening pollutant in food, herein, we developed a Tb3+-nucleic acid probe-based label-free assay for mix-and-read, rapid detection of mercury pollution. The assay utilized the feature of light-up fluorescence of terbium ions (Tb3+) via binding with single-strand DNA. Mercury ion, Hg2+ induced thymine (T)-rich DNA strand to form a double-strand structure (T-Hg2+-T), thus leading to fluorescence reduction. Based on the principle, Hg2+ can be quantified based on the fluorescence of Tb3+, the limit of detection was 0.0689 μmol/L and the linear range was 0.1 - 6 μmol/L. Due to the specificity of T-Hg2+-T artificial base pair, the assay could distinguish Hg2+ from other metal ions. The recovery rate was ranged in 98.71% -101.34% for detecting mercury pollution in three food samples. The assay is low-cost, separation-free and mix-to-read, thus was a competitive tool for detection of mercury pollution to ensure food safety.
| Original language | English |
|---|---|
| Pages (from-to) | 1-11 |
| Journal | Food Science and Human Wellness |
| Volume | 13 |
| DOIs | |
| Publication status | Published - 14 Apr 2023 |
Bibliographical note
Note: This work was financially supported by National Natural Science Foundation of China (grant no: 22074100), the Young Elite Scientist Sponsorship Program by CAST (grant no: YESS20200036), the Researchers Supporting Project Number (grant no: RSP-2021/138), King Saud University, Riyadh, Saudi Arabia, and Technological Innovation R&D Project of Chengdu City (grant no: 2019-YF05-317 02266-SN) and Sichuan University- Panzhihua City joint Project (grant no: 2020CDPZH-5).Keywords
- Agriculture, veterinary and food science