Tb 3+-nucleic acid probe-based label-free and rapid detection of mercury pollution in food

Xuhan Xia; Chenxi Zhou; Yulin Zhu; Yi Dong; Qiang He; Mohammad Rizwan Khan; Yuanlong Chi; Rosa Busquets; Ruijie Deng; Yao Ren

doi:10.26599/FSHW.2022.9250085

Tb 3+-nucleic acid probe-based label-free and rapid detection of mercury pollution in food

Xuhan Xia
, Chenxi Zhou
, Yulin Zhu
, Yi Dong
, Qiang He
, Mohammad Rizwan Khan
, Yuanlong Chi
, Rosa Busquets
, Ruijie Deng
, Yao Ren

Research output: Contribution to journal › Article › peer-review

Abstract

Mercury is a threatening pollutant in food, herein, we developed a Tb3+-nucleic acid probe-based label-free assay for mix-and-read, rapid detection of mercury pollution. The assay utilized the feature of light-up fluorescence of terbium ions (Tb3+) via binding with single-strand DNA. Mercury ion, Hg2+ induced thymine (T)-rich DNA strand to form a double-strand structure (T-Hg2+-T), thus leading to fluorescence reduction. Based on the principle, Hg2+ can be quantified based on the fluorescence of Tb3+, the limit of detection was 0.0689 μmol/L and the linear range was 0.1 - 6 μmol/L. Due to the specificity of T-Hg2+-T artificial base pair, the assay could distinguish Hg2+ from other metal ions. The recovery rate was ranged in 98.71% -101.34% for detecting mercury pollution in three food samples. The assay is low-cost, separation-free and mix-to-read, thus was a competitive tool for detection of mercury pollution to ensure food safety.

Original language	English
Pages (from-to)	1-11
Journal	Food Science and Human Wellness
Volume	13
DOIs	https://doi.org/10.26599/FSHW.2022.9250085
Publication status	Published - 14 Apr 2023

Bibliographical note

Note: This work was financially supported by National Natural Science Foundation of China (grant no: 22074100), the Young Elite Scientist Sponsorship Program by CAST (grant no: YESS20200036), the Researchers Supporting Project Number (grant no: RSP-2021/138), King Saud University, Riyadh, Saudi Arabia, and Technological Innovation R&D Project of Chengdu City (grant no: 2019-YF05-317 02266-SN) and Sichuan University- Panzhihua City joint Project (grant no: 2020CDPZH-5).

Keywords

Agriculture, veterinary and food science

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10.26599/FSHW.2022.9250085

https://doi.org/10.26599/FSHW.2022.9250085

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title = "Tb 3+-nucleic acid probe-based label-free and rapid detection of mercury pollution in food",

abstract = "Mercury is a threatening pollutant in food, herein, we developed a Tb3+-nucleic acid probe-based label-free assay for mix-and-read, rapid detection of mercury pollution. The assay utilized the feature of light-up fluorescence of terbium ions (Tb3+) via binding with single-strand DNA. Mercury ion, Hg2+ induced thymine (T)-rich DNA strand to form a double-strand structure (T-Hg2+-T), thus leading to fluorescence reduction. Based on the principle, Hg2+ can be quantified based on the fluorescence of Tb3+, the limit of detection was 0.0689 μmol/L and the linear range was 0.1 - 6 μmol/L. Due to the specificity of T-Hg2+-T artificial base pair, the assay could distinguish Hg2+ from other metal ions. The recovery rate was ranged in 98.71\% -101.34\% for detecting mercury pollution in three food samples. The assay is low-cost, separation-free and mix-to-read, thus was a competitive tool for detection of mercury pollution to ensure food safety.",

keywords = "Agriculture, veterinary and food science",

author = "Xuhan Xia and Chenxi Zhou and Yulin Zhu and Yi Dong and Qiang He and Khan, \{Mohammad Rizwan\} and Yuanlong Chi and Rosa Busquets and Ruijie Deng and Yao Ren",

note = "Note: This work was financially supported by National Natural Science Foundation of China (grant no: 22074100), the Young Elite Scientist Sponsorship Program by CAST (grant no: YESS20200036), the Researchers Supporting Project Number (grant no: RSP-2021/138), King Saud University, Riyadh, Saudi Arabia, and Technological Innovation R\&D Project of Chengdu City (grant no: 2019-YF05-317 02266-SN) and Sichuan University- Panzhihua City joint Project (grant no: 2020CDPZH-5).",

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doi = "10.26599/FSHW.2022.9250085",

language = "English",

volume = "13",

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journal = "Food Science and Human Wellness",

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T1 - Tb 3+-nucleic acid probe-based label-free and rapid detection of mercury pollution in food

AU - Xia, Xuhan

AU - Zhou, Chenxi

AU - Zhu, Yulin

AU - Dong, Yi

AU - He, Qiang

AU - Khan, Mohammad Rizwan

AU - Chi, Yuanlong

AU - Busquets, Rosa

AU - Deng, Ruijie

AU - Ren, Yao

N1 - Note: This work was financially supported by National Natural Science Foundation of China (grant no: 22074100), the Young Elite Scientist Sponsorship Program by CAST (grant no: YESS20200036), the Researchers Supporting Project Number (grant no: RSP-2021/138), King Saud University, Riyadh, Saudi Arabia, and Technological Innovation R&D Project of Chengdu City (grant no: 2019-YF05-317 02266-SN) and Sichuan University- Panzhihua City joint Project (grant no: 2020CDPZH-5).

PY - 2023/4/14

Y1 - 2023/4/14

N2 - Mercury is a threatening pollutant in food, herein, we developed a Tb3+-nucleic acid probe-based label-free assay for mix-and-read, rapid detection of mercury pollution. The assay utilized the feature of light-up fluorescence of terbium ions (Tb3+) via binding with single-strand DNA. Mercury ion, Hg2+ induced thymine (T)-rich DNA strand to form a double-strand structure (T-Hg2+-T), thus leading to fluorescence reduction. Based on the principle, Hg2+ can be quantified based on the fluorescence of Tb3+, the limit of detection was 0.0689 μmol/L and the linear range was 0.1 - 6 μmol/L. Due to the specificity of T-Hg2+-T artificial base pair, the assay could distinguish Hg2+ from other metal ions. The recovery rate was ranged in 98.71% -101.34% for detecting mercury pollution in three food samples. The assay is low-cost, separation-free and mix-to-read, thus was a competitive tool for detection of mercury pollution to ensure food safety.

AB - Mercury is a threatening pollutant in food, herein, we developed a Tb3+-nucleic acid probe-based label-free assay for mix-and-read, rapid detection of mercury pollution. The assay utilized the feature of light-up fluorescence of terbium ions (Tb3+) via binding with single-strand DNA. Mercury ion, Hg2+ induced thymine (T)-rich DNA strand to form a double-strand structure (T-Hg2+-T), thus leading to fluorescence reduction. Based on the principle, Hg2+ can be quantified based on the fluorescence of Tb3+, the limit of detection was 0.0689 μmol/L and the linear range was 0.1 - 6 μmol/L. Due to the specificity of T-Hg2+-T artificial base pair, the assay could distinguish Hg2+ from other metal ions. The recovery rate was ranged in 98.71% -101.34% for detecting mercury pollution in three food samples. The assay is low-cost, separation-free and mix-to-read, thus was a competitive tool for detection of mercury pollution to ensure food safety.

KW - Agriculture, veterinary and food science

U2 - 10.26599/FSHW.2022.9250085

DO - 10.26599/FSHW.2022.9250085

M3 - Article

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VL - 13

SP - 1

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JO - Food Science and Human Wellness

JF - Food Science and Human Wellness

ER -

Tb 3+-nucleic acid probe-based label-free and rapid detection of mercury pollution in food

Abstract

Bibliographical note

Keywords

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