Visual pigment reconstitution in intact goldfish retina using synthetic retinaldehyde isomers

Juliet W.L. Parry; James K. Bowmaker

doi:10.1016/s0042-6989(00)00101-2

Visual pigment reconstitution in intact goldfish retina using synthetic retinaldehyde isomers

Juliet W.L. Parry
, James K. Bowmaker

University College London

Research output: Contribution to journal › Article › peer-review

Abstract

A protocol has been developed for reconstituting visual pigments in intact retinae by delivering synthetic isomers of retinal incorporated in phospholipid vesicles. Calibration curves have been constructed relating the λmax of the native porphyropsins (visual pigments based on 11-cis 3-dehydroretinal) of the rods and four spectral classes of cone in the goldfish, and the equivalent photosensitive pigments regenerated from 11-cis retinal (rhodopsins) and the commercially available isomer, 9-cis retinal (isorhodopsins). The relationship between the λ_max of rhodopsins and isorhodopsins appears to be linear, such that the difference in λ_max changes sign at about 380 nm. We therefore conclude that the protocol for reconstituting visual pigments with 9-cis retinal is suitable for all classes of vertebrate opsin-based photopigments.

Original language	English
Pages (from-to)	2241-2247
Number of pages	7
Journal	Vision Research
Volume	40
Issue number	17
Early online date	31 Jul 2000
DOIs	https://doi.org/10.1016/s0042-6989(00)00101-2
Publication status	Published - Aug 2000
Externally published	Yes

Keywords

Chromophore
Goldfish
Isorhodopsin
Retinaldehyde
Visual pigment

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10.1016/s0042-6989(00)00101-2Licence: Other

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title = "Visual pigment reconstitution in intact goldfish retina using synthetic retinaldehyde isomers",

abstract = "A protocol has been developed for reconstituting visual pigments in intact retinae by delivering synthetic isomers of retinal incorporated in phospholipid vesicles. Calibration curves have been constructed relating the λmax of the native porphyropsins (visual pigments based on 11-cis 3-dehydroretinal) of the rods and four spectral classes of cone in the goldfish, and the equivalent photosensitive pigments regenerated from 11-cis retinal (rhodopsins) and the commercially available isomer, 9-cis retinal (isorhodopsins). The relationship between the λmax of rhodopsins and isorhodopsins appears to be linear, such that the difference in λmax changes sign at about 380 nm. We therefore conclude that the protocol for reconstituting visual pigments with 9-cis retinal is suitable for all classes of vertebrate opsin-based photopigments.",

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TY - JOUR

T1 - Visual pigment reconstitution in intact goldfish retina using synthetic retinaldehyde isomers

AU - Parry, Juliet W.L.

AU - Bowmaker, James K.

PY - 2000/8

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N2 - A protocol has been developed for reconstituting visual pigments in intact retinae by delivering synthetic isomers of retinal incorporated in phospholipid vesicles. Calibration curves have been constructed relating the λmax of the native porphyropsins (visual pigments based on 11-cis 3-dehydroretinal) of the rods and four spectral classes of cone in the goldfish, and the equivalent photosensitive pigments regenerated from 11-cis retinal (rhodopsins) and the commercially available isomer, 9-cis retinal (isorhodopsins). The relationship between the λmax of rhodopsins and isorhodopsins appears to be linear, such that the difference in λmax changes sign at about 380 nm. We therefore conclude that the protocol for reconstituting visual pigments with 9-cis retinal is suitable for all classes of vertebrate opsin-based photopigments.

AB - A protocol has been developed for reconstituting visual pigments in intact retinae by delivering synthetic isomers of retinal incorporated in phospholipid vesicles. Calibration curves have been constructed relating the λmax of the native porphyropsins (visual pigments based on 11-cis 3-dehydroretinal) of the rods and four spectral classes of cone in the goldfish, and the equivalent photosensitive pigments regenerated from 11-cis retinal (rhodopsins) and the commercially available isomer, 9-cis retinal (isorhodopsins). The relationship between the λmax of rhodopsins and isorhodopsins appears to be linear, such that the difference in λmax changes sign at about 380 nm. We therefore conclude that the protocol for reconstituting visual pigments with 9-cis retinal is suitable for all classes of vertebrate opsin-based photopigments.

KW - Chromophore

KW - Goldfish

KW - Isorhodopsin

KW - Retinaldehyde

KW - Visual pigment

U2 - 10.1016/s0042-6989(00)00101-2

DO - 10.1016/s0042-6989(00)00101-2

M3 - Article

C2 - 10927111

AN - SCOPUS:0033651036

SN - 0042-6989

VL - 40

SP - 2241

EP - 2247

JO - Vision Research

JF - Vision Research

IS - 17

ER -

Visual pigment reconstitution in intact goldfish retina using synthetic retinaldehyde isomers

Abstract

Keywords

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